We need to extract lipids from sunflower seeds for UPLC-MS. Which protocol is better to take?
Thank you!
aronEnlightened
Best way for lipid extortion from sunflower seeds?
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We need to extract lipids from sunflower seeds for UPLC-MS. Which protocol is better to take?
Thank you!
John Wick
usually for plant seeds, we utilised the Soxhlet method with ethyl ether as a solvent to obtain a crude lipid extract while when we performed a fat extraction for GC fatty acid determination we utilised a (3:2) mixture of hexane isopropanol. Francesco
We used the Soxhlet method for oil extraction from Moringa oliefera seed oil. You can try this method.
avery
A very precise investigation of the effects of temperature and contact time on extraction efficiency of sunflower cake was reported using hexane as solvent (Baümler ER et al., JAOCS 2010, 87, 1489). Extraction at 60°C during 30 min leads to a very high yield (99%) for triacylglycerols and tocopherols and to a reduced phospholipid extraction (66%).
Andrew Pel
there is two methods for extracting oil from oleaginous seeds: chemical method using solvent (hexane) with soxhlet apparatus or mechanic method using press extractor