line transect method
Is it important to select the direction of the transect (NEWS) while sampling butterflies?
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The direction of the transect does not matter. It is important to avoid the edge effect when selecting the position of your transects and the distance of the transects must be the same, for example 50 or 100 meters inside your plots so that the abundance and diversity data can be compared for different habitats.
I think it is better to consider the transect directions. Because transect direction will also influence your sampling. You need to find the factors that influencing the distribution of butterflies as said above by others. Then only the line transect is more meaningful and that is the fundamental to line transect. I feel you understand the fact that I mentioned.
Yes, But it must add that in some biotopes, a transect of for instance 50 m., is in part at the shadow of trees or of bushes. At these places, the distribution of butterflies varies with the species and also with the preference of species of butterflies according to time in the day and to temperature in the season.
As a very general rule, it is not. After all the ‘transect’ is going to be the same, whatever the direction (sense) is. However there might be practical issues, such as the position of the sun early in the morning or late in the evening, or when there are any dominating winds… these are practical details that one has to determine in each case, or this is my guess at least.